Hepa 1-6

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

Hepa 1-6 (mouse, liver, hepatoma)

IZSBS catalog code: BS TCL 157
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: mouse
TISSUE/ORGAN: liver
TUMOR: hepatoma
DEPOSITOR: obtained from ATCC
PROPERTIES: secreting several liver-specific products (such as albumin, alpha-fetoprotein, alpha 1-antitrypsin and amylase)

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
GMEM + 10% FBS + 2mM L-Glutamine + 100 units/ml DIA/LIF + 0.1mM 2-mercaptoethanol; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, mycoplasma negative, culture
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: CRL-1830, ECACC: 92110305, DSMZ: ACC 175

Bibliographic references:

J Natl Cancer Inst 1980;64:809-819 - PMID: 6102619
Methods Enzymol 1987;151:19-38 - PMID: 3431441