RPMI 1846

Version 4.0.200811

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RPMI 1846 (hamster, golden Syrian, skin, melanoma, melanotic)

SPECIES: hamster, golden Syrian
TISSUE/ORGAN: skin
TUMOR: melanoma, melanotic

ECACC catalog code: 93120834
Morphology: Epithelial
Description: Hamster Syrian melanotic melanoma
Viruses: See Properties
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia, ITALY
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 10-20% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 1.5x1,000,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 27; Karyotype: 2n = 44
Derived from a spontaneous malignant melanoma in an aged golden Syrian hamster. The cells have the ability to produce melanin in vitro. The TD50 for new-born hamsters is 68 cells.
Research council deposit: No; Price Code: C;

Availability in cell line catalogues: IZSBS: BS TCL58