|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, AKR/J
TISSUE/ORGAN: blood
TUMOR: lymphoma, T cell
ECACC catalog code: 88100507
Morphology: Lymphoblast
Description: Mouse AKR/J T cell lymphoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Resistance and hybridization studies
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100µM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1588
Bibliographic references:
- J Natl Cancer Inst 1974;52:429 - PMID: 4363715
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it