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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, AKR/J
TISSUE/ORGAN: blood
TUMOR: lymphoma
ECACC catalog code: 85082302
Morphology: Lymphoblast
Description: Mouse AKR/J T cell lymphoma
Viruses: See Properties
Depositor: Dr P Marrack/Dr J Kappler, National Jewish Center, Denver, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37C.
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100µM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- Cell 1978;14:21 - PMID: 667934
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it