A549

Version 4.0.200811

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A549 (human, lung, carcinoma)

IZSBS catalog code: TCL 101
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: human Karyology: 2n = 46 in 40 cells
TISSUE/ORGAN: lung
TUMOR: carcinoma
DEPOSITOR: obtained from ATCC
PROPERTIES: producing lecithin; expressing xenobiotic metabolizing enzymes

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
Ham's F12K + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 77, mycoplasma negative, culture
Expressing differentiated functions of alveolar type II cells
Validated by: isoenzymes (confirmed as human with NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: CCL-185, ECACC: 86012804, ICLC: HTL03001

Bibliographic references:

Int J Cancer 1976;17:62-70 - PMID: 175022
J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758