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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse/mouse
TUMOR: hybridoma
DSMZ catalog code: ACC 183
Morphology: single cells growing in suspension
Description: mouse hybridoma (anti-adenovirus 2) established by fusion of SP2/0-AG14 myeloma cells with spleen cells from BALB/c mice immunized with HELA cells that were infected with adenovirus 2; cells secrete an IgG monoclonal antibody reacting with an adenovirus group specific antigen; cell line is patented confirmed as mouse with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS optimal split ratio of 1:2 every 2-3 days at 37C with 5% CO2 cell harvest of about 1-2 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine karyotype with centric fusion and 16% polyploidy: 66(58-74)<3n>) * isoenzymes (confirmed as mouse with IEF of AST, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HB 8117
Bibliographic references:
- Virology 1981;110:385-401 - PMID: 7222481
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it