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ECACC catalog code: 97041101
Description: Rat lymphoma
Viruses: See Properties
Depositor: Dr Peter Gout and Dr Henry Friesen, Department of Cancer Endocrinology, British Columbia Cancer Agency, Vancouver, CANADA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Modified prolactin receptor (the Nb2 form) Applications: 1)In vitro bioassay of lactogenic hormones. 2)Studies of the mechanisms of lactogen induced mitogenesis. 3)Studies into the action of vinca alkaloids. 4)Studies of tumour progression.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Fischer's medium + 10%Foetal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Na Bicarbonate + 0.05mM 2-Mercaptoethanol (2ME) + 2mM Glutamine. Seed cultures at 4x1000 and 12x1000 cells/ml for culture periods of 96 and 72 hours respectively. Do not exceed 9x100,000 cells/ml. Avoid using dilutions of cells with fresh medium at greater than 1:10 ratios; culture doubling time 12 hours.
Nb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from ECACC on request.
Research council deposit: No;
Price Code: C;
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293