BUD-8

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

BUD-8 (human, Caucasian, skin, fibroblast)

SPECIES: human, Caucasian
TISSUE/ORGAN: skin, fibroblast

ECACC catalog code: 90031532
Morphology: Fibroblast
Description: Human Caucasian skin fibroblast
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Interferon assay

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 7; Karyotype: Diploid
Derived from the normal skin of a 56 year old Caucasian female. The cells are highly sensitive to human interferon and have been proposed as a standard for its assay.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1554, IZSBS: BS CL11

Bibliographic references:

J Biol Stand 1979;7:383 - PMID: 536379