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TUMOR: carcinoma, cervical
ECACC catalog code: 85022104
Description: Human cervical carcinoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transfection studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA. 5% CO2; 37C. Passage every 2-3 days when confluent at 1:3 dilution.
HeLa derivative rendered TK- by selection in BUdR.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Int J Cancer 1966;1:19 - PMID: 5902315
- Virology 1970;5:446
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293