N1E-115

Version 4.0.200811

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N1E-115 (mouse, A/Jax, nervous system, neuroblastoma)

SPECIES: mouse, A/Jax
TISSUE/ORGAN: nervous system
TUMOR: neuroblastoma

ECACC catalog code: 88112303
Morphology: Neuronal
Description: Mouse neuroblastoma
Viruses: See Properties
Depositor: Dr Nirenberg, Laboratory of Biochemical Genetics, National Institute of Health, Bethesda, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 30; Adrenergic clone derived from the mouse neuroblastoma C1300 tumour.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 2263

Bibliographic references:

Proc Natl Acad Sci USA 1972;69:258-263 - PMID: 4400294