BT-474

Version 4.0.200811

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BT-474 (human, breast, carcinoma, ductal)

SPECIES: human
TISSUE/ORGAN: breast
TUMOR: carcinoma, ductal

DSMZ catalog code: ACC 64
Morphology: adherent patches of epithelial cells; individual colonies with a thick center and a thin smooth margin
Susceptibility to viruses: RIII-MuMTV
Description: human breast ductal carcinoma established from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman; cells were reportedly tumorigenic in athymic mice and were found to be susceptible to mouse mammary tumor virus confirmed as human with IEF of AST, GPDH, LDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS + 10 æg/ml bovine insulin + 2 mM L-glutamine split confluent culture 1:2 to 1:3 every 7-10 days using trypsin/EDTA; cells grow slowly; seed out at ca. 2-3 x 106 cells/25 cm 2 at 37C with 5% CO2 about 1.0-1.6 x 105 cells/cm 2 or 20 x 106 cells/175 cm 2; doubling time of ca. 100 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (Hypertetraploid karyotype; 104(91-111)<4n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, GPDH, LDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HTB-20, ICLC: HTL00008

Bibliographic references:

In Vitro 1979;15:723-729 - PMID: 94035
J Natl Cancer Inst 1978;61:967-978 - PMID: 212572