380

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

380 (human, peripheral blood, leukemia, pre-B cell)

ICLC catalog code: HTL99019
Continuous culture, grown as suspension
round, small cells morphology
SPECIES: human (male, 15 years old) Karyology: (8;14) and (14;18) translocations
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, pre-B cell
Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse
DEPOSITOR: Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: EBNA negative

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 1-2 x 10^6 cells/ml; split 1:2-1:3; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
the cells are very difficult to culture, grow very slowly and should be frozen at not less than 5 x 10^6 cells/ampoule
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: DSMZ: ACC 39

Bibliographic references:

Proc Natl Acad Sci USA 1984;81:7166-7170 - PMID: 6334305