ACHN

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

ACHN (human, Caucasian, kidney, adenocarcinoma)

ICLC catalog code: HTL99007
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 22 years old)
TISSUE/ORGAN: kidney
TUMOR: adenocarcinoma
Derived from: pleural effusion
tumorigenic in nude mice
DEPOSITOR: Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: antiproliferative studies, using human interferons or interferon inducers

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR - mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: IZSBS: BS TCL68, ECACC: 88100508

Bibliographic references:

Cancer Res 1987;47:4582-4589 - PMID: 2957045