|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL98005
Continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human
TUMOR: neuroblastoma
DEPOSITOR: Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
THIS CELL LINE IS NOT PRESENTLY AVAILABLE FOR DISTRIBUTION AT THIS LABORATORY
-
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:4-1:8 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
the cells detach very easily (one or two min.)
Validated by: isoenzymes (confirmed as human with MD, NP)
Bibliographic references:
- Cancer Res. 1977;37(5):1364-1371 - PMID: 856461
- Cancer Res. 1977;37(5):1372-1376 - PMID: 15722
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it