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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL98016
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: mouse, C57L
TISSUE/ORGAN: liver
TUMOR: hepatoma, BW7756
Derived from: Hepa-1cl, cloned from the Hepa-1 cell line
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells possess the aryl hydrocarbon (Ah) receptor and are highly inducible for cytochrome P4501A1; drug metabolism assays
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, Pep B)
Bibliographic references:
- Biochimie 1991;73:61-66 - PMID: 1851644
- Dev Biol 1973;35:83-96 - PMID: 4362668
- Proc Natl Acad Sci USA 1979;76:373-376 - PMID: 106390
- Science 1980;209:497-499 - PMID: 6248960
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it