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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
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ICLC catalog code: ATL98014
Continuous culture, grown as monolayer
epithelial; the cells grow in large clusters morphology
SPECIES: rat
TISSUE/ORGAN: liver
TUMOR: hepatoma
Derived from: transplantable Morris hepatoma (n.7795, Buffalo strain)
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells synthesize and secrete serum albumin, C9, tyrosine aminotransferase; monooxigenases expression; toxicity screening; xenobiotic metabolism; drug metabolism assays; hepatocyte studies
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-2 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
cell harvest is about 10 x 10^6 cells/25 cm2 flask
Validated by: isoenzymes (confirmed as rat with AST, MD)
Availability in cell line catalogues: ECACC: 85112702, DSMZ: ACC 106
Bibliographic references:
- Cell Biochem Funct 1988;6:245-250 - PMID: 3191583
- Exp Cell Res 1991;196:330-336 - PMID: 1716586
- J Cell Biol 1969;40:236-247 - PMID: 4177659
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it