A8/DI

Version 4.0.200811

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A8/DI (mouse, AKR, thymus, thymoma)

ICLC catalog code: ATL98012
Continuous culture, grown as suspension
lymphoblast morphology
SPECIES: mouse, AKR
TISSUE/ORGAN: thymus
TUMOR: thymoma
Original line: AKR/1a murine T cell line
TRANSFORMED BY: exposed to a dose of 500 rads from an X-ray source Back To Radiant Agents' WebPage
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: useful in the study of assembly and transport of Lyt-2/3 antigen in the cell surface or to define the regions of Lyt-2/3 interacting with the corresponding antibodies and with MHC class I molecules

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; maintain cultures between 3-9x10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with AST, MD)

Availability in cell line catalogues: ECACC: 94031011

Bibliographic references:

Immunogenetics 1994;40(2):154-158 - PMID: 7517914
J Immunol Methods 1992;154(2):211-216 - PMID: 1401954