|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human
TISSUE/ORGAN: kidney, embryonic
ECACC catalog code: 92052131
Morphology: Suspension
Description: Human embryo kidney
Viruses: See Properties
Depositor: Dr F L Graham, Department of Biology, McMaster University, Ontario, CANADA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Adenovirus 5 early region 1(E1) antigens
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Joklik's MEM + 2mM Glutamine + 10% Horse Serum (HS). Maintain cultures between 5x10,000 to 5x100,000 cells/ml in a Techne stirrer 5% CO2; 37C. Cell doubling time is 30 hours.
Adapted for growth in suspension by passaging 293 cells (ECACC No. 85120602) in nude mice. The cells express adenovirus 5 early region 1(E1) antigens and is permissive for adenovirus 5 host range mutants defective in E1 functions.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- J Gen Virol 1987;68:937 - PMID: 3819703
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it