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SPECIES: mouse, (BALB/cxNZB) F1
TUMOR: lymphoma, lymphoblastic
ECACC catalog code: 85022107
Description: Mouse B cell lymphoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Surface Immunoglobulin M (IgM) Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:8 to 1:10 i.e. seeding at 2-9x100,000 cells/ml; 5% CO2; 37C.
Expresses surface IgM but does not secrete Ig. Secretion of IgM can be induced with LPS. Wehi 231 is a B cell lymphoma of BALB/c x NXB F1 origin induced by mineral oil injection.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Immunology 1980;39:57-60 - PMID: 6966604
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
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Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293