WEHI-3B

Version 4.0.200811

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WEHI-3B (mouse, BALB/c, leukemia, myelomonocytic)

SPECIES: mouse, BALB/c
TUMOR: leukemia, myelomonocytic

DSMZ catalog code: ACC 26
Morphology: small, round, single cells growing adherently and partly in suspension
Description: mouse myelomonocytic leukemia macrophage-like cells derived from a BALB/c mouse treated for tumor induction; cells produce IL-3 confirmed as mouse with IEF of LDH, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. E. Schmitt, Institute of Immunology, University of Mainz, Mainz, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% Iscove's MDM + 5-10% FBS + 2 mM L-glutamine + 2.5 x 10-5 M mercaptoethanol split confluent culture 1:2 to 1:5 twice to three times weeky; cells are trypsin-sensitive, detach mechanically after loosening by incubating in PBS (without Ca 2+ or Mg 2+ ) for 10-15 min; seed out at ca. 0.2-0.5 x 105 cells/cm 2 at 37C with 5% CO2 maximum cell harvest of about 1 x 106 cells/cm 2; doubling time of ca. 20-30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1.0-1.5 x 106 cells/ampoule; negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid karyotype with 19% polyploidy; 73/74(70-79)<4n>; 2-3 centric fusion markers present) * isoenzymes (confirmed as mouse with IEF of LDH, NP)

Availability in cell line catalogues: ICLC: ATL99003

Bibliographic references:

Cancer Res 1977;37:546-550 - PMID: 318922
J Cell Physiol 1982;110:101-104 - PMID: 7068764
J Immunol 1977;119:950-954 - PMID: 894031
J Immunol 1982;128:2393 - PMID: 6122701
J Natl Cancer Inst 1969;43:963 - PMID: 5259325
Proc Natl Acad Sci U S A 1984;81:1208 - PMID: 6422470