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SPECIES: hamster, golden Syrian
ECACC catalog code: 84100501
Susceptibility to viruses: adenovirus 25 * arbovirus * foot and mouth disease virus * herpes simplex * poliovirus * rabies * vesicular stomatitis
Description: Hamster Syrian kidney
Viruses: See Properties
Depositor: Mr P Radlett/Dr M Draper, Coopers Animal Health, Pirbright, Surrey, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus replication studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 44, pseudodiploid
BHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (ECACC No. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 10
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293