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SPECIES: mouse, Tg transgenic
ECACC catalog code: 95062833
Morphology: Polymorph, clumps of small cells
Description: Mouse pancreatic small cell anaplastic carcinoma
Viruses: See Properties
Depositor: Prof OS Pettengill and Dr DS Longnecker, Dartmouth Medical School, Lebanon, NH, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Somatostatin and Insulin Applications: Study of exocrine pancreas carcinomas, hormone secretion studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2150
- Carcinogenesis 1994;15:61-65 - PMID: 7904904
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293