TB1 Lu (NBL-12)

Version 4.0.200811

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TB1 Lu (NBL-12) (bat, lung)

SPECIES: bat
TISSUE/ORGAN: lung

ECACC catalog code: 90020805
Morphology: Epithelial
Susceptibility to viruses: rabies * reovirus 3 * vaccinia * vesicular stomatitis (Ogden strain)
Description: Bat lung
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: rabies virus

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 69; Karyotype: 2n = 48
Derived from the lung of an adult female bat. Rabies virus produces CPE in this cell line. The cells have been demonstrated to propagate for 20 more passages beyond passage 69 as supplied.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 88

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it