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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: human, Caucasian
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma, grade III
ECACC catalog code: 91030714
Morphology: Epithelial
Description: Human Caucasian colon adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA) Applications: Tumourigenicity studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L-15 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; No CO2; 37C.
Passages: 55;
Derived from a grade III adenocarcinoma of the colon of an 81 year old Caucasian female. The cells synthesise relatively large amounts of CEA (2,000ng/1,000,000 cells in 21 days).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 237
Bibliographic references:
- Cancer Res 1979;36:4562-4569 - PMID: 1000501
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it