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TUMOR: leukemia, Friend
ECACC catalog code: 91112122
Description: Mouse Friend leukaemic lymphoblast
Viruses: See Properties
Depositor: Dr Y A Wilkinson, Biomedical Sciences Research Centre, University of Ulster, Coleraine, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
SF12 + 2mM Glutamine + 1% AA + 1.05g Sodium Bicarbonate (NaHCO3) + 4ml (1M) NaOH + 10% Horse Serum (HS). Maintain cultures between 3-6x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Chromosome Nos 33-41
Derived from CLONE 707. Resistant to 30ug/ml BUdR. TK deficient. Increased chemical and physical mutagen susceptibility.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293