BGM

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

BGM (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

ECACC catalog code: 90092601
Morphology: Fibroblast
Susceptibility to viruses: Coxsackie * echovirus * many types of viruses * poliovirus * reovirus * rotavirus
Description: Monkey African green kidney
Viruses: See Properties
Depositor: Dr S Clark, CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: polio, Echo, Coxsackie and Reo viruses

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:8 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin EDTA; 5% CO2; 37C.
Passages: 80; Used for the isolation of water borne viruses e.g. polio 1, 2, 3, Echo 3, 6, 7, 9, 12, 27, Coxsackie A9 + B1, 2, 3 and Reo 1.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: IZSBS: BS CL 7

Bibliographic references:

Append Environ Microbiol 1986;51:790
Arch Gesamte Virusforsch 1970;32:389 - PMID: 4993582
Health Lab Sci 1974;110:275 - PMID: 4369820