SK-MES-1

Version 4.0.200811

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SK-MES-1 (human, lung, carcinoma, squamous cell)

ICLC catalog code: HTL95004
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (male, 65 years old) Karyology: hypotriploid
TISSUE/ORGAN: lung
TUMOR: carcinoma, squamous cell
Derived from: pleural effusion
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: HLA: A3, Aw30; B7, B27

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (HBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 3-5x104 cells/cm2. 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, MPI)

Availability in cell line catalogues: IZSBS: BS TCL72, DSMZ: ACC 353

Bibliographic references:

Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
In Vitro 1975;115
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871