SH-SY5Y

Version 4.0.200811

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SH-SY5Y (human, neuroblastoma)

SPECIES: human
TUMOR: neuroblastoma

DSMZ catalog code: ACC 209
Morphology: epithelial-like cells growing as monolayer and in cell clusters (when cultured at high density); cells do not grow to complete confluency
Description: human neuroblastoma clonal subline of the neuroepithelioma cell line SK-N-SH that had been established in 1970 from the bone marrow biopsy of a 4-year-old girl with metastatic neuroblastoma confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Heikkilae, University of Turku, Turku, Finland
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-85% Dulbecco's MEM + 15-20% FBS split culture 1-2 times a week using trypsin/EDTA; seed out with 2 x 106 cells/80 cm 2 at 37C with 5-10% CO2 cell harvest of about 15 x 106 cells/175 cm 2; doubling time of >55 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human near-diploid karyotype with 1.8% polyploidy; 46/47(42-48)<2n>X/XX, +7, ins(1) (q32q11q43), add(9)(q34), der(22)t(?17;22)(q22;q13)) * isoenzymes (confirmed as human with IEF of AST, MDH)

Availability in cell line catalogues: ECACC: 94030304, ICLC: HTL95013

Bibliographic references:

Cancer Res 1973;33:2643-2652 - PMID: 4748425
Cancer Res 1978;38:3751-3757 - PMID: 29704
Cancer Res 1990;50:3422-3428 - PMID: 2334938