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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
S+L-CAT2 (cat)
ICLC catalog code: ATL96011
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: cat
TRANSFORMED BY: murine sarcoma virus
DEPOSITOR: Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: virus replication; virology; the cell line does not produce murine leukemia virus (L-).
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 or DMEM + 2 mM L-Glutamine + 10% FBS; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2. 37C, 5% CO2. Passage: 7, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as cat with AST, G6PD)
Bibliographic references:
- J Gen Virol 1983;64:425-428 - PMID: 6300292
- J Virol 1974;14:177-179 - PMID: 4365331
- Virology 1975;67:288-291 - PMID: 170737
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it