BF-2

Version 4.0.200811

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BF-2 (fish - bluegill fry, caudal trunk)

SPECIES: fish - bluegill fry
TISSUE/ORGAN: caudal trunk

ECACC catalog code: 87032603
Morphology: Fibroblast
Description: Fish Bluegill fry caudal trunk
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies, targets for bacterial cytotoxins

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) (pH7.4 - 7.5). Split confluent cultures 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 23C. A replication capacity of up to 50 passages is expected.
Passages: 48; Karyotype: 2n = 48
Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15C to 33C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 91, IZSBS: BS CL 102

Bibliographic references:

J Infect Dis 1968;11:253 - PMID: 5674065
J Virol 1968;2:393 - PMID: 4986903
Science 1966;151:1004 - PMID: 4955835