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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: rat, Sprague-Dawley
TISSUE/ORGAN: endometrium
ECACC catalog code: 92092513
Morphology: Spindle shaped
TRANSFORMED BY: SV40
Description: Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed
Viruses: See Properties
Depositor: Prof M Beato, Institut fur Molekularbiologie and Tumorforschung, W-3550 Marburg/Lahn, GERMANY
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Glucocorticoid
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 20µg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Fetal Bovine Serum (FBS) (heat-inactivated). Split confluent cultures 1:5 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Doubling time 35 h.
Passages: 14;
Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, connexin 43, desmin, proenkephalin, uteroglobin and uvomorulin markers.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Bibliographic references:
- Oncogene 1990;5:787 - PMID: 1694289
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it