RD

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

SPECIES: human, Caucasian
TISSUE/ORGAN: muscle
TUMOR: rhabdomyosarcoma, embryonal

ECACC catalog code: 85111502
Morphology: Spindle-shaped
Susceptibility to viruses: Coxsackie A * enterovirus * herpes simplex 1 * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
Description: Human Caucasian embryo rhabdomyosarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 40; Karyotype: Modal No 56
Derived from a malignant embryonal rhabdomyosarcoma from a 7 year old Caucasian female. Also designated TE 32 and 130T in the literature
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 136

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326