RD

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

RD (human, Caucasian, muscle, rhabdomyosarcoma, embryonal)

Continuous culture, grown as monolayer
spindle-shaped morphology
SPECIES: human, Caucasian (female, 7 years old) Karyology: modal number 50;microchromosomes, chr. association
TISSUE/ORGAN: muscle
TUMOR: rhabdomyosarcoma, embryonal
Susceptibility to viruses: Coxsackie A * enterovirus * herpes simplex 1 * poliovirus 1 * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: protein secr.; myoglobin ATPase activity; myosin ATPase activity; genetics; nucleic acids; tumorigenicity; differentiation; cell biology; protein

Available in the following LABORATORY:

Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% Antibiotics + 1% non-essential amino acids; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-4, mycoplasma negative, DAPI and HOECHST
Cloning efficiency <1%. Split twice a week
Validated by: membrane markers

Availability in cell line catalogues: ATCC: CCL 136, ECACC: 85111502

Bibliographic references:

Cancer 1969;24:520 - PMID: 4241949
J Clin Microbiol 1990;28:1049 - PMID: 2161864
J Virol 1967;1:326