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SPECIES: human, Caucasian
ECACC catalog code: 95011816
Morphology: Rounded to fusiform growing in aggregates
Description: Human Caucasian neuroblastoma
Viruses: See Properties
Depositor: Dr R A Ross and Dr BA Spengler, Department of Biological Sciences, Fordham University, New York, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of neuroblastoma
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS):Ham's F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:50 i.e. seeding at 4x10,000 cells/cm2 using 0.1% trypsin or PBS only; 5% CO2; 37C.
The cell line BE(2)-M17 was isolated from SK-N-BE(2) (ECACC No. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hrs, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-ß-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cancer Res 1989;49:219 - PMID: 2535691
- Cancer Res 1993;53;4978 - PMID: 8402688
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293