PSN1

Version 4.0.200811

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PSN1 (human, pancreas, adenocarcinoma)

SPECIES: human
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma

ECACC catalog code: 94060601
Morphology: Epithelial
Description: Human pancreatic adenocarcinoma
Viruses: See Properties
Depositor: Dr T Sekiya, National Cancer Center, Research Institute, 1-1 Tsukiji 5-Chome, Chuo-ku, Tokyo 104, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: PSN1 is an example of a cancer cell having multiple genetic changes including those of two oncogenes (c-Ki-ras and c-myc) and a tumour suppresser gene (p53).

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 7; The human pancreatic cancer cell line PSN1 was derived from pancreatic adenocarcinoma tissue. After transplantation into nude mice the PSN1 cell line was established from the xenograft. PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ICLC: HTL97008

Bibliographic references:

Biochem Biophys Res Commun 1986;140:167 - PMID: 3778442
Cancer Res 1991;51:3356 - PMID: 2054775