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ICLC catalog code: ATL97002
Continuous culture, grown as monolayer
TISSUE/ORGAN: brain, fetal
Original line: G355
TRANSFORMED BY: Moloney-MSV
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: virus replication; viral diagnostics; the cells can detect and assay type C retroviruses and are currently recommended by the FDA for detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use
Available in the following LABORATORY:
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
McCoy's 5A + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. Passage: 26, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as cat with G6PD, MD.)
- J Virol 1985;53:827-833 - PMID: 2983093
- Virology 1982;123:139-151 - PMID: 6959413
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293