PC-12

Version 4.0.200811

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PC-12 (rat, adrenal, pheochromocytoma)

SPECIES: rat
TISSUE/ORGAN: adrenal
TUMOR: pheochromocytoma

DSMZ catalog code: ACC 159
Morphology: small cells growing in clumps in suspension, adhering poorly to plastic
Description: rat adrenal pheochromocytoma established from a transplantable rat adrenal pheochromocytoma in 1976; cells were described to synthesize catecholamines (dopamine, norepinephrine); in response to nerve growth factor (NGF) a neuronal phenotype could be induced reversibly confirmed as rat with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from ATCC (CRL 1721), Rockville, Maryland, USA
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85% RPMI 1640 + 10% horse serum + 5% FBS split saturated culture 1:2 to 1:3 every 3-4 days; cells grow in suspension without collagen; using collagen (Collagen S- Roche at 30 æg/ml) cells grow adherent, faster and to a higher density; the adherent cells may be detached mechanically without trypsin, for single cells the suspension should be passed several times through a syringe with a 22-gauge needle; seed out at ca. 5-15 x 106 cells/25 cm 2 at 37C with 5% CO2 about 1-2 x 106 cells/ml; doubling time of ca. 50-60 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as rat with IEF of MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CRL 1721, ECACC: 88022401, ICLC: ATL98004, IZSBS: BS TCL 91

Bibliographic references:

EMBO J 1983;2:643 - PMID: 6641712
Methods Enzymol 1984;1147:207
Proc Natl Acad Sci U S A 1976;73:2424-2428 - PMID: 1065897
Science 1985;229:393-395 - PMID: 3839317