P3X63Ag8U.1

Version 4.0.200811

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P3X63Ag8U.1 (mouse, BALB/c, plasmacytoma)

SPECIES: mouse, BALB/c
TUMOR: plasmacytoma

ECACC catalog code: 94120802
Morphology: Lymphoblast
Description: Mouse Myeloma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: This cell line is useful for fusion to spleen cells to produce hybridomas or for hybridization studies with other myelomas or lymphomas. Can also be used for immunoglobulin production.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Maintain cultures at 5-9x100,000 cells/ml; 5% CO2: 37C.
Passages: +3; P3x63Ag8U.1 is a derivative of the P3x63Ag8 cell line (ECACC 85011401) from the mouse myeloma MOPC21, which was derived from a female BALB/c mouse. This variant no longer synthesises gamma-1 chains but still produces kappa light chains which are not secreted.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1597, ICLC: ATL96012

Bibliographic references:

Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
J Exp Med 1979;150:580 - PMID: 90108