This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, BALB/c
ECACC catalog code: 90030803
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
Description: Mouse BALB/c embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and transformation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS). Split confluent cultures 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.1% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 40, hypotetraploid
Derived from a 14-17 day old BALB/c mouse embryo. The cells are tumorigenic, are insensitive to contact inhibition and are susceptible to transformation with SV40 virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 164
- J Cell Physiol 1968;72:141-148 - PMID: 4301006
- Science 1968;162:1024-1026 - PMID: 4301647
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293