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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse
TUMOR: myeloma
ECACC catalog code: 85011417
Morphology: Lymphoblast
Description: Mouse myeloma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner, hybridization and tumourigenicity studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.
Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridization studies with other myelomas or lymphomas.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species ocnfirmation)
Availability in cell line catalogues: ATCC: CRL 1597
Bibliographic references:
- Curr Top Microbiol Immunol 1978;81:1-7 - PMID: 567551
- J Exp Med 1979;150:580 - PMID: 90108
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105737-289, Fax: +39-0105737-293
Email: assunta.manniello@istge.it