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SPECIES: mouse, DBA/2
ECACC catalog code: 87121104
Description: Mouse DBA/2 mastocytoma
Viruses: See Properties
Depositor: Dr Van Pel, Ludwig Institute for Cancer Research, Brussels, BELGIUM
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Genetic, transfection and tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 8% CO2; 37C.
From P815 after serial cycles of transfection. This HTR variant shows high efficiency of transfection (0.00001) for neomycin-resistance and TK gene or other exogenous DNA. Possible to select TK- cells with BUdR for resubmission to transfection with TK gene.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Somat Cell Mol Genet 1977;11:467
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293