NRK-49F

Version 4.0.200811

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NRK-49F (rat, kidney, fibroblast)

SPECIES: rat
TISSUE/ORGAN: kidney, fibroblast

DSMZ catalog code: ACC 172
Morphology: fibroblast-like cells with a fusiform shape
Description: rat kidney fibroblasts cell line was cloned from a mixed culture of normal rat kidney cells; cells exhibit contact inhibition and are very sensitive; indicator cells for viral or chemical transformation; this cell line is frequently used for TGF-ss bioassays; (= ATCC CRL 1570) confirmed as rat with IEF of AST, NP, PEP B
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
95% Dulbecco's MEM + 5% FBS split culture 1:4 to 1:5 about 2-3 times a week using trypsin/EDTA when the cells have reached 3/4 of confluence at 37C with 5% CO2 cell harvest of about 3.5 x 106 /80 cm 2; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid rat karyotype with 4% polyploidy; 40(37-42)<2n>) * isoenzymes (confirmed as rat with IEF of AST, NP, PEP B)

Availability in cell line catalogues: ATCC: CRL 1570, ECACC: 86101301

Bibliographic references:

J Bacteriol 1966;92:1133-1140 - PMID: 5926742
J Cell Physiol 1978;94:335 - PMID: 304450