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SPECIES: rat, Osborn-Mendel
TISSUE/ORGAN: kidney, fibroblast
ECACC catalog code: 86032002
Susceptibility to viruses: vesicular stomatitis
Description: Rat kidney fibroblast
Viruses: See Properties
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus titration
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from normal rat kidney (Rattus norvegicus). The cells are useful for focus-forming assays in virus titrations.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Int J Cancer 1973;12:654 - PMID: 4134008
- J Bacteriol 1966;92:1133-1140 - PMID: 5926742
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293