NRK

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

NRK (rat, Osborn-Mendel, kidney, fibroblast)

SPECIES: rat, Osborn-Mendel
TISSUE/ORGAN: kidney, fibroblast

ECACC catalog code: 86032002
Morphology: Fibroblast
Susceptibility to viruses: vesicular stomatitis
Description: Rat kidney fibroblast
Viruses: See Properties
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus titration

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from normal rat kidney (Rattus norvegicus). The cells are useful for focus-forming assays in virus titrations.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Bibliographic references:

Int J Cancer 1973;12:654 - PMID: 4134008
J Bacteriol 1966;92:1133-1140 - PMID: 5926742