NIH/3T3

Version 4.0.200811

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NIH/3T3 (mouse, NIH Swiss, embryo)

Monoclonal continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: embryo
Susceptibility to viruses: herpes simplex * murine leukemia virus * murine sarcoma virus * N-tropic oncornaviruses C * vaccinia * vesicular stomatitis
PROPERTIES: contact inhibition; transformation; transfection; cell biology

Available in the following LABORATORY:

Consorzio Mario Negri SUD, Centro di Ricerche Biomediche e Farmacologiche (CHIBC, S.Maria Imbaro (CH))
DMEM (with 4.5 g/l glucose) + 10% Calf serum; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3 Passage: 133, mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790