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SPECIES: monkey, marmoset
ECACC catalog code: 85011419
Morphology: Lymphoblast and Fibroblast
TRANSFORMED BY: Epstein-Barr
Endogenous viruses: contains Epstein-Barr
Description: Monkey Marmoset peripheral blood lymphocyte
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Epstein Barr Virus (EBV) Applications: Transformation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml. May need to use 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Releases high titres of transforming EBV. Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1612, DSMZ: ACC 100
- Proc Natl Acad Sci U S A 1973;70:190-194 - PMID: 4346033
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293