B95-8

Version 4.0.200811

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B95-8 (monkey, marmoset, blood)

SPECIES: monkey, marmoset
TISSUE/ORGAN: blood

ECACC catalog code: 85011419
Morphology: Lymphoblast and Fibroblast
TRANSFORMED BY: Epstein-Barr
Endogenous viruses: contains Epstein-Barr
Description: Monkey Marmoset peripheral blood lymphocyte
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Epstein Barr Virus (EBV) Applications: Transformation studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml. May need to use 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Releases high titres of transforming EBV. Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells should be handled under laboratory containment level 2.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1612, DSMZ: ACC 100

Bibliographic references:

Proc Natl Acad Sci U S A 1973;70:190-194 - PMID: 4346033