NCI-H727

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

NCI-H727 (human, Caucasian, lung, carcinoma, non small cell)

SPECIES: human, Caucasian
TISSUE/ORGAN: lung
TUMOR: carcinoma, non small cell

ECACC catalog code: 94060303
Morphology: Epithelial
Description: Human lung non-small cell carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Synthesize the peptide neuromedin B (NMB) (0.1 pmol/mg protein). Applications: Synthesis of NMB.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 57; A non-small cell carcinoma of the lung from a 65 year old Caucasian female taken, prior to treatment. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. The cells are not able to synthesise gastrin releasing peptide (GRP). NCI-H727 is the best differentiated available bronchial carcinoid cell line. Any proposed commercial use of these cells or products must first be negotiated with the University of Texas. More details available upon request.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 5815, ICLC: HTL97001

Bibliographic references:

Cancer Res 1992;52 (Suppl):2732-2736
Science 1989;246:491-494 - PMID: 2554494