NC-37

Version 4.0.200811

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NC-37 (human, Caucasian, blood)

SPECIES: human, Caucasian
TISSUE/ORGAN: blood

ECACC catalog code: 89111414
Morphology: Lymphoblast
Endogenous viruses: contains Epstein-Barr
Description: Human Caucasian peripheral blood lymphocyte
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: EBV, oncornavirus propagation

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 4-5x100,000 cells/ml; Maximum cell density is 2x1,000,000 cells/ml; 5% CO2; 37C.
Karyotype: 2n = 46
Derived from peripheral blood of a normal 34 year male Caucasian. Each cell has an average 60 EBV genome copies. Cells have been used in EBV superinfection studies in addition to chemical induction of the EBV genome. They are EBNA positive. The cells should be handled under laboratory containment level 2.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 214

Bibliographic references:

Int J Cancer 1970;6:436 - PMID: 4321017