NAMALWA

Version 4.0.200811

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NAMALWA (human, African, lymphoma, Burkitt)

SPECIES: human, African
TUMOR: lymphoma, Burkitt

DSMZ catalog code: ACC 24
Morphology: lymphoblastoid, single cells or small clusters in suspension
Description: human Burkitt's lymphoma established from the tumor mass of an African child with Burkitt's lymphoma in 1967; cells express c-myc mRNA confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at about 2.0 x 106 cells/ml or higher; doubling time of about 20-30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypodiploid with 4% polyploidy, a supernumerary ring or fragment present in 8% of cells; 44(43-46)<2n>X, -Y -5, -6, +7, -10, -13, -21, +3m, ins(1;?)(p11;?), ins(3)(q11q29), der(5)t(1;5)(q25;q35), del(8)(p22), der(14)t(14;?) * cytogenetics 2 (der(15)t(15;21)(p11;q11); NAMALWA marker chromosomes present) * DNA fingerprinting (HinfI-(gtg)5 DNA profile=NAMALWA subclones, otherwise unique) * immunological analysis (CD3- CD13- CD19+ CD37+ DR+ cy/sIgM+) * isoenzymes (confirmed as human with IEF of AST, NP)

Availability in cell line catalogues: ATCC: CRL 1432, IZSBS: BS TCL 47, ECACC: 87060801

Bibliographic references:

Antimicrob Agents Chemother 1979;15:420 - PMID: 465469
Cancer 1969;23:64-79 - PMID: 4178827
Dev Biol Stand 1978;38:343
Int J Cancer 1972;10:44 - PMID: 4122458
Int J Cancer 1973;12:396 - PMID: 4365093
J Gen Virol 1982;63:207 - PMID: 7175502