B9

Version 4.0.200811

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B9 (mouse/mouse, hybridoma)

SPECIES: mouse/mouse
TUMOR: hybridoma

DSMZ catalog code: ACC 211
Morphology: round cells growing in suspension
Description: mouse hybridoma cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer; cells are IL6-dependent confirmed as mouse with IEF of AST, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. L. Aarden, Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS + 50 覡 mercaptoethanol + 50 pg/ml human IL-6 split saturated culture 1:3 to 1:4 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml, best in 24- or 6-well plate at 37C with 5% CO2 maximal density at about 0.8 x 106 cells/ml; doubling time of ca. 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (apparently murine chromosome morphology with extensive centric fusions) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ICLC: HYL98004, ECACC: 96080128

Bibliographic references:

Eur J Immunol 1987;17:1411-1416 - PMID: 3500054