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SPECIES: rat, Fisher
ECACC catalog code: 85103116
Description: Rat Fischer fibroblast
Viruses: See Properties
Depositor: Mrs M Freshney, The Beatson Institute, Glasgow, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Vitamins + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from a subclone of a BUdR-resistant strain of a Fischer rat fibroblast 3t3-like line RAT-1. The line is suitable for transformation and TGF (SGF) assays as an indicator cell line.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Virology 1981;113:408-411 - PMID: 7269249
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293